The NCERT Solutions for Class 12 Biotechnology Chapter 4 Applications of Recombinant DNA Technology cover all 29 exercise questions, according to the latest 2026-27 NCERT textbook. Each answer explains DNA fingerprinting, transgenic organisms, gene therapy, recombinant vaccines and therapeutic agents in simple exam language.
- Question count: 29 solved NCERT exercise questions with assertion and MCQ answer checks.
- Best for: board revision, biotechnology viva practice and one-shot chapter recap.
- PDF includes: stepwise solutions, expert explanations and boxed final answers.
Student Feedback: Students revising this chapter said the MCQs became easier after grouping DNA fingerprinting, vaccines, Humulin and antibody questions by application.
Class 12 Biotechnology Chapter 4 Applications of Recombinant DNA Technology Solutions PDF
Class 12 Biotechnology Chapter 4 Applications of Recombinant DNA Technology NCERT Solutions help students revise how recombinant DNA methods solve real problems. The chapter covers DNA fingerprinting, transgenic organisms, gene therapy, vaccines, Humulin, growth hormone and humanised antibodies.
Most students lose marks when they mix up a tool with an application. The PDF separates each process into concept, steps and final answer.
How to Use These Applications of rDNA Technology NCERT Solutions
Start with DNA fingerprinting and transgenic plants because they build the base. Then revise gene therapy and vaccines. Finish with Humulin, Humatrope, recombinant antibodies and the MCQ set.
- Use the boxed answer for last-minute recall.
- Read the expert answer when an assertion-reason item feels close.
- Revise the comparison tables before school tests.
DNA Fingerprinting Revision Map
DNA fingerprinting depends on VNTR polymorphism in non-coding DNA. RFLP-based analysis follows a fixed order: isolate DNA, cut it with restriction enzymes, separate fragments, transfer them to a membrane, hybridise with a VNTR probe and compare the band pattern.
| Step | What Happens | Why It Matters |
|---|---|---|
| Restriction digestion | DNA is cut into fragments | Creates length differences |
| Electrophoresis | Fragments separate by size | Builds the band pattern |
| Southern transfer | DNA moves to membrane | Prepares it for probing |
| VNTR probe | Probe binds matching repeats | Reveals individual-specific bands |
Transgenic and Vaccine Applications
Transgenic plants are made by adding a useful gene and regenerating transformed cells into a full plant. Recombinant vaccines use a cloned antigen gene. Therapeutic proteins such as Humulin and recombinant growth hormone are produced by expressing human genes in host cells.
Important MCQ Answers in Applications of rDNA Technology
The important MCQ answers are: B cells plus myeloma cells for hybridoma technology, non-coding sequences for DNA fingerprinting, probe for complementary detection, Bacillus thuringiensis for cry genes, non-heritable for somatic gene therapy and Humulin for recombinant insulin.
A small student feedback check showed that the assertion-reason items became easier after students wrote the exact NCERT reason beside each assertion.
Applications of Recombinant DNA Technology Video Revision
Source: YouTube classroom revision video
Check All Applications of rDNA Technology Exercise Answers
The solved question cards below let students open the main solution and expert solution separately.
Related Class 12 Biotechnology Resources
| Resource | Use it for |
|---|---|
| Applications of Recombinant DNA Technology NCERT Book PDF | Read the official chapter before solving. |
| Gene Cloning NCERT Solutions | Revise the previous recombinant DNA workflow. |
| Genome Technology and Engineering NCERT Solutions | Move to the next chapter after revision. |
All Class 12 Biotechnology NCERT Solutions
| Chapter | NCERT Solutions |
|---|---|
| Chapter 1 An Overview of Recombinant DNA Technology | Open Chapter 1 Solutions |
| Chapter 2 Host-Vector System | Open Chapter 2 Solutions |
| Chapter 3 Gene Cloning | Open Chapter 3 Solutions |
Applications of Recombinant DNA Technology Biotechnology NCERT Solutions FAQs
Ques. What is covered in Class 12 Biotechnology Chapter 4 Applications of Recombinant DNA Technology NCERT Solutions?
Ans. The solutions cover DNA fingerprinting, RFLP, VNTR probes, GMOs, transgenic plants, gene therapy, recombinant vaccines, Humulin and antibodies.
Ques. How many questions are solved in Chapter 4 Applications of Recombinant DNA Technology?
Ans. The PDF solves all 29 NCERT exercise questions, including descriptive questions, assertion-reason items, MCQs and fill-in-the-blank questions.
Ques. What is the correct answer for recombinant insulin?
Ans. Genetically engineered insulin is called Humulin. It is produced by recombinant DNA technology using bacterial expression of insulin chains.
Ques. What is the main basis of DNA fingerprinting?
Ans. DNA fingerprinting mainly depends on polymorphic non-coding repeat sequences such as VNTRs, which differ between individuals.
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