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Tissue can be defined as a group of cells that perform a shared function that is similar in shape and size.
- In other words, tissue possesses a similar structure that functions together.
- They are not present in unicellular organisms and are poorly differentiated among the simplest multicellular species, such as sponges.
- Now, you are done with knowing the definition of tissue so let’s quickly go through its classification.
Here, we are going to study tissue and diversity in the shapes and sizes of plants and animal cells through an experiment but before going further let us first be aware of some theoretical concepts like tissue and cells and their further classification.
| Table of Content |
Key Terms: Tissue, Cells, Types of tissues, Animal cell, Plant cell, Unicellular organisms, Multicellular species, Parenchyma
Aim of the Experiment
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To study the tissues and variety of shapes and sizes of plant and animal cells such as palisade cells, parenchyma, Sclerenchyma, Collenchyma, Sclerenchyma, xylem, phloem, mammalian blood smear, squamous epithelium, and muscle fibers through the training of permanent slide.
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Theory
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What is a Tissue?
A tissue is a group of cells that perform a function that is similar in size and shape.
Tissues can be grouped as follows:
Simple Tissue
It consists of only one type of cell. They are further sub-categorized into-
- Parenchyma
- Collenchyma
- Sclerenchyma
Complex Tissue
They have more than one type of cell.
- Meristematic tissue
- Permanent tissue
The further classification of permanent tissue includes Simple permanent tissue, complex permanent tissue, and secretory tissue.

Requirements
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The following are the requirements to perform the experiment
Permanent slide of the following:
- T.S of lotus leaf, T.S of Nerium leaf, and T.S of lotus stem or petiole.
- Vertical section (V.S) of the shoot apex and root apex.
- Macerated material of Bougainvillea, Tridax.
For Maceration Technique:
- Small twigs of any locally accessible plant
- Safranine
- Glycerine
- Beaker (100ml) or boiling test tube
- Some Slides
- Glass rod
- Cotton blue for staining
- Tripod Stand
- Burner
- Microscope with adjusted lens
- Knife (sharp-edged)
- Wire gauge
- Thread
- Muslin cloth
For Maceration fluid:
- Chromic acid should be properly dissolved with an equal quantity of 10% nitric acid. (caution: handle any acid properly)
- For preparing chromic acid you need concentrated H2SO4 (sulphuric acid) gradually dissolved in 100 ml of water.
- Now, it’s time to add K2Cr2O7 (i.e., potassium dichromate) – 50 gm
- Your stock solution is absolutely ready. Now, dilute 10 ml of this solution up to 100 ml so that it can be usable for the experimentation process. Your working solution for the maceration is now ready.
Procedure
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The following are the procedures of the experiment
Step 1
You need some fine green and fresh branches of woody plants from your locality. For thickness purposes, you can refer to the thickness of the toothpick.
Step 2
Cut the twigs into smaller bits of approx. 0.5 cm long (Be careful when you are holding any sharp-edged instruments).
Step 3
Put the cut pieces of twigs in the beaker filled with water. Make arrangements for boiling i.e., on the tripod stand, place the wire gauge, and switch on the burner. Boil the sample for at least 10- 15 minutes.
Step 4
Then transfer the sample into another beaker in which you have already put the maceration fluid. Start boiling it until it turns soft and pulpy for 10-15 minutes.
Step 5
Put some drops of Safranine in the material in order to stain the xylem and the phloem.
Step 6
Now place the stained material in a drop of glycerine into a slide.
Step 7
Carefully, split the cells on the slide itself with the help of two needles.
Step 8
Put the coverslip onto the slide and just observe under the well-set microscope.
Analyze the following slides:
- For Aerenchyma – T.S of lotus petiole.
- For spongy tissue and palisade- T.S of Nerium leaf
- For the meristem- V.S of the shoot and root apex
- For simple tissues- T.S of Cucurbita stem or Menthe stem.
Observations
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- In order to see the Aerenchyma you need to observe the large intercellular space in the cells.
- Chorenchyma is a parenchyma cell with chloroplasts usually columnar and compactly aligned cells.
- The epidermis or protective tissue is the parenchyma tissue.
- Sclerenchyma will be pointed by lacking intercellular spaces and elongated in shape.
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Things to Remember
- Tissue can be defined as a group of cells that work together to perform a specific function.
- These cells typically share similar shapes and sizes.
- Tissues can be classified as simple tissues and complex tissues.
- Chlorenchyma, a type of parenchyma tissue, is composed of cells containing chloroplasts.
- These cells can be arranged in two main ways: spongy (loosely packed) or palisade (columnar and compactly aligned).
Sample Questions
Ques. Name a plant type where we can find aerenchyma cells. (1 Mark)
Ans. Aerenchyma cells are found in aquatic plants such as Hydrilla.
Second Answer: Aerenchyma is a spongy tissue that develops air channels within the leaves, stems, and roots of halophytic plants. These air channels facilitate the exchange of gases between the shoot and root systems.
Ques. Write about the constituency of the sclerenchyma cell wall. (1 Mark)
Ans. Sclerenchyma cell walls have thick cell walls containing lignin with high levels of cellulose content.
Second Answer: Sclerenchyma cells are characterized by two types of cell walls: primary and secondary. The secondary wall, which is much thicker and heavily lignified (15%–35%), provides significant rigidity and hardness to the cell and surrounding tissue. There are two main types of sclerenchyma cells: fibers and sclereids.
Ques. Differentiate between Sclerenchyma, Collenchyma, and parenchyma. (3 Marks)
Ans. The differences between Sclerenchyma, Collenchyma, and parenchyma are given in the table below
| Properties | Parenchyma | Sclerenchyma | Collenchyma |
|---|---|---|---|
| Cell wall | Thin | Thick | Thick primary cells at the corners |
| Intercellular spaces | Many | Absent | Absent |
| Nature of cells | Living and nucleus present | Dead | Living and nucleus present |
| Shape | Oval, polygonal, spherical, rod-shaped | Elongated | Variable shape |
Second Answer: Ground tissue in plants consists of three main types of simple permanent tissues: parenchyma, collenchyma, and sclerenchyma.
- Parenchyma: It is found throughout the organs of the plants such as roots, stems, leaves, flowers, fruits, and seeds. Parenchyma is a versatile ground tissue that primarily functions as filler tissue in soft plant parts. Examples include the cortex and pith of stems, the cortex of roots, the mesophyll of leaves, the pulp of fruits, and the endosperm of seeds.
- Collenchyma: In herbaceous dicotyledonous stems, collenchyma generally appears as a three- to four-layered hypodermis. It is found mainly near the vascular cambium, a type of outward-growing tissue. It provides structural support to growing shoots and leaves. For example, the stringy texture of celery stalks is due to the presence of collenchyma cells.
- Sclerenchyma: Sclerenchyma fibers are mainly associated with the xylem and phloem of vascular bundles. They occur in various plant parts: the hypodermis of monocot stems, the pericycle of many dicots, secondary wood, the vascular bundle sheath in monocot stems, and the hypodermis of many leaves.
Ques. What are the types of animal tissues and describe the functions it performs? (4 Marks)
Ans. There are four types of Animal tissues – Epithelial tissue, Muscle tissue, Nervous tissue, and connective tissue.
- Epithelial tissue: It consists of layers of compactly packed cells that line the surface of the body generally for protection, secretion, and absorption.
- Muscle tissue: It is made up of cells containing contractile filaments that move past each other and change their shapes and sizes.
- Nervous tissue: It is made up of the nerve cells or the neurons that all together form the nervous system, including the spinal cord and brain.
- Connective tissue: It can either be loosely or densely packed and is involved in the structure and support of the body.
Second Answer: Animal cells are organized into various tissues. These tissues differ in structure, function, and developmental origin. There are four main types of animal tissues: epithelial tissue, connective tissue, muscle tissue, and nervous tissue.
Epithelial tissues are classified as Sensory epithelium, Glandular epithelium, Pigmented epithelium, and Absorptive epithelium.
Connective tissues are classified as Connective tissue proper, Vascular tissue, and Skeletal tissue.
Muscle tissues are classified as Cardiac, Smooth, and Skeletal.
Ques. What is the composition of Sclerenchyma tissue? (2 Marks)
Ans. Sclerenchyma is simple permanent tissue generally elongated in shape and the cell wall is thick consisting of lignin and a high level of cellulose content. However, the vacuole is absent in the sclerenchymatous tissue. They are typically dead cells and their wall consists of a few to several pits.
Second Answer: Mature sclerenchyma tissue is composed of dead cells with heavily thickened walls rich in lignin and cellulose (60%–80%). These dead cells provide structural support throughout the plant. Sclerenchyma cells have two types of cell walls: primary and secondary walls.
Ques. What is a cell? Differentiate between plant and animal cells? (5 Marks)
Ans. A cell is a basic building block or it is a structural, functional, and biological unit of all living organisms. The differences between plant and animal cells are given below:
| Features | Plant cells | Animal cells |
|---|---|---|
| Cell shapes | Square or rectangular in shape | Generally irregular or round in shape |
| Cell wall | Present | Absent |
| Plasma/cell membrane | Present | Present |
| Endoplasmic reticulum | Present | Present |
| Nucleus | Present and lies on one side of the cell | Present and lies generally in the center of the cell |
| Vacuoles | Large and centrally placed | Usually small and numerous |
| Mitochondria | Present but fewer in number | Present and are numerous in nature |
Second Answer: In biology, the cell is the fundamental unit of life. This membrane-bound structure contains the essential molecules for life and forms the building block of all living things. Some organisms, like bacteria or yeast, exist as single cells. In multicellular organisms like humans and animals, cells differentiate into specialized types as they mature. These specialized cells work together to form tissues, organs, and the entire organism.
The smallest known cells are mycoplasmas, a group of tiny bacteria. Some of these single-celled spheres can be as small as 0.2 micrometers (μm) in diameter, roughly equivalent to 0.000039 inches. In comparison, human cells are about 400,000 times more massive than a single mycoplasma bacterium. However, even human cells are microscopic, typically measuring around 20 μm across.
Ques. What are the types of muscle tissue found in animal cells? (2 Marks)
Ans. Muscle tissue is a highly specialized tissue found in animal cells. It is characterized by its ability to contract and relax, enabling movement. It consists of muscle fibers that help in contraction and relaxation. There are basically 3 types of Muscle tissue-
- Smooth muscle tissue: Found in the inner lining of the organs.
- Skeletal muscle tissue: Attached to the bones.
- Cardiac muscle tissue: Found in the heart only.
Second Answer: Muscle tissue is a highly cellular tissue and well-supplied with blood vessels. It is composed of specialized cells that possess the remarkable ability to shorten or contract, generating the force necessary for body movement.
Ques. What is the study of plant and animal cells called? (1 Mark)
Ans. The study of plant and animal cells is called cytology.
Second Answer: Cytology comes from the Greek words "cyto" meaning cell and "logos" meaning study. It is a branch of biology that focuses on the structure, function, and behavior of cells.
Ques. Which is the smallest cell? (1 Mark)
Ans. Mycoplasma gallisepticum is considered one of the smallest cells, measuring only 0.2–0.3 micrometers (μm) in size. In fact, Mycoplasma are the smallest known living organisms on Earth.
Second Answer: Mycoplasmas are a group of bacteria that are extremely small. They are considered to be the smallest self-replicating organisms known to exist. They do not have a cell wall, which is a rigid structure that surrounds most bacteria and gives them their shape.
Ques. Which is the longest animal cell? (1 Mark)
Ans. The longest animal cell is a nerve cell, also called a neuron. The length of these cells can be up to 3 feet (1 meter).
Second Answer: A nerve cell, also called a neuron, is a specialized cell found in the nervous system that transmits electrical signals. These signals are essential for coordinating involuntary and voluntary actions, sensations, thoughts, and emotions throughout the body.
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