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Colloidal solutions are insoluble particles of another mixture. If you look in the colloidal solutions in-depth, you will get the dispersed particles of another substance. This is the reason it is also called Colloid. The egg Albumins come from the eggs that form lyophilic sols when mixed with cold water. If the mixtures and the impurities are stable, the sols will not get disturbed and will have the medium phase of dispersants. The same can be depicted from a simple experiment.
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Aim
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To prepare a colloidal solution of egg albumin
Requirements
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- Funnel
- Filter paper
- Burner
- Wire gorge
- Soluble starch of 500ML
- Tripod stand
- Distilled water
- Glass Rod
- Beakers of 250 ml and 50ML
- Pestle and Mortar
- Porcelain Dish
Check Important Types of Solutions
Procedure
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- Break down the hard shell of the egg using the glass road of the apparatus.
- You will see the yellow part and the colorless liquid in the form of albumin water. You need to color that albumin water and transfer the colorless liquid into another beaker.
- Take a new beaker of 250ML and pour around 100 ML of NaCl solution which must be equal to 5% of the solution.
- Burst the albumin and mix it with the initial solution or sodium chloride solution that you have prepared in that 250ML beaker.
- Continuously stir the solution or the mix for 20 minutes unless you are sure that the egg albumins are prepared in the water and the sols are also made.
- Once the solutions are segregated, filter the substance into a fresh beaker using filter paper and a funnel.
- Once the filtration is done, label the same as easily as possible so that the proper identification can be made in the future.
Check Important Notes for Principle of Homogeneity
Precautions
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- When using the apparatus for the experiment make sure that the same is cleaned with distilled water and you have dried it properly.
- While preparing the egg albumin sol never uses normal water. Instead, always prefer using distilled water for the same.
- Prepare the egg albumin sol at room temperature.
- Segregate the album in a yolk only after mixing the same with sodium chloride and not before such mix.
- The egg albumin sol will not be prepared in the beaker if the mix is not constantly stirred for 20 minutes.
Things to Remember
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- The colorless liquid within the egg is called egg albumin and the yellow part is known as the yolk.
- The albumin must be mixed with the distilled water otherwise the sols will not be prepared.
- Hot water does not have a good reaction to the creation of lyophilic sol. Therefore only cold water is used to have a stable and unaffected reaction of the impurities.
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Sample Questions
Ques. What are the requirements for experimenting with preparing the lyophilic sols of egg albumin? (3 Marks)
Ans. The Requirements for the experiment are-
- Funnel
- Filter paper
- Burner
- Wire gorge
- Soluble starch of 500ML
- Tripod stand
- Distilled water
- Glass Rod
- Beakers of 250 ml and 50ML
- Pestle and Mortar
- Porcelain Dish
Ques. How do get the dispersed colloidal particles in the solution? (3 Marks)
Ans. The egg albumin when taken out from the original egg should be added slowly and smoothly with the solution in the beaker. Continuous stirring is a must. If the same is followed then the colloidal particles will show their dispersed reaction in the solution only and the filtration will become an easy process.
Ques. Discuss the characteristics of colloidal solutions. (3 Marks)
Ans.
- The size of the same varies from 1 nm to 1000nm.
- In sols, dispersions are seen in different mediums and different phases. Majorly it is the solid medium.
- When the particles of another substance are found in the solution, the colloids are formed.
Ques. What are the different types of dispersion sols? (3 Marks)
Ans. Colloidal solutions are divided mainly into two types-
Lyophobic Sols
These particles have a low to medium dispersion that has no interaction with the other phase of sols. The affinity of this medium is different from the other phase and thus has the dispersed phase of the medium. It does not have many groupings with the liquid and therefore is known as the liquid hating sols.
Lyophilic Sols
These solvents have good coordination with the liquid and they get dissolved in such liquid very easily. The medium is firmly attached to the solution and thus the phase is dispersed solvents.
Ques. Discuss the importance of cold water while preparing the egg albumin. (3 Marks)
Ans. Coldwater will help in transferring into the sols. It helps in maintaining the stability of the nature of egg albumin. The traces of impurities will not affect the experiment. If the hot water is used it will disperse the mediums of the souls and the egg album will lose its stability. Instead of water one can also look for form because it is another form of good dispersed medium.
Ques. What are the different kinds of colloidal systems? (5 Marks)
Ans. There are mainly five kinds of colloidal systems.
Foam- The form is the first type where the dispersed phase is mainly the gas. And the medium used is either solid or liquid. For example, one can use whipped cream or bubble bath as the medium and dispersed phase.
Aerosol- Aerosol is the second form of Colloidal system that has the dispersed phase of either solid or liquid. The dispersion medium used is gas. Here one can use either perfume or fog as their medium.
Gel- The gel is the third and the mean kind of colloidal system that has the solid dispersion phase and the medium used is liquid. An example of such a combination is rubber, toothpaste, cheese, and even gelatin.
Sol- Sol is the 4th form of colloidal system that can be extracted from this experiment. The dispersion phase used here is solid while the medium is liquid. For example mud, blood, and paint can be used as such a medium.
Emulsion- The emulsion is another form of colloidal system that has liquid as dispersion phase and the medium used here is also liquid. For example milk, coffee can be used as medium and phase.
Ques. Explain Colloids or Colloidal solutions.(3 Marks)
Ans. Colloidal solutions are insoluble particles of another mixture. If you look in the colloidal solutions in-depth, you will get the dispersed particles of another substance. This is the reason it is also called Colloid. In sols, dispersions are seen in different mediums and different phases. Majorly it is the solid medium.
Ques. What are the methods of colloids purification? (3 Marks)
Ans. The solutions obtained from such preparation are impure. The impurities can disturb the colloids and the suspended particles. The electrolytes are generally small in concentration and thus stabilize the sol. If the concentration is higher than the sol does not get prepared. Therefore, colloids solution must be pure or if impure it must be purified by using electrolyte effects. Some common methods used for such verification are dialysis, electrodialysis, ultracentrifugation, and ultrafiltration.
Ques. Briefly explain the process of segregating an egg yolk and egg albumin in a solution. (3 Marks)
Ans. Separating the egg yolk from the egg albumin is the major and primary step in the extraction. First, the egg's outer shell needs to be broken in a proper dish and the colorless album is supposed to be taken out from the mixture. Always prefer using a narrow tube for extracting the liquid into different beakers. It will also help in measuring the liquids which you are extracting. If the sodium chloride is mixed with the album and stirred for a long 20 minutes then the extraction process becomes easier.
Ques. Whether albumin is found in the human body? (3 Marks)
Ans. Yes, albumin is also found in the blood of the human body and also in the milk if consumed. If the milk is concentrated, then the albumin in the form of lactose can be observed. Albumin is generally prepared by the liver of the human body. It helps in maintaining the colloidal pressure In the blood and prevents the plasma. The albumin also helps in transporting the molecules within the human body such as fatty acids, hormones, and various drugs.
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