Microbes as Biofertilizers: Rhizobium, Nostoc and Azotobacter

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Jasmine Grover

Education Journalist | Study Abroad Lead

Microbes as biofertilizers increase the nutrient availability to crop plants either directly or through soil enrichment. These are scientifically made and distributed to farmers. The use of biofertilizers is advantageous mainly because it is environmentally friendly and sustainable. Nowadays, the use of biofertilizers is encouraged over chemical fertilizers, since these don’t disturb the ecological balance.

Key Takeaways: Biofertilizer, Microbes, Azotobacter, Anabaena, Nostoc, Crop plants, Fertilizer, Soil, Microorganisms, Bacteria


Biofertilizers

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Biofertilizers are microorganisms which enrich the soil with nutrients, maximize the ecological benefits and minimize environmental impact.

The microorganisms are cultivated in scientific laboratories in suitable culture media. The cultured microorganism is autoclaved to destroy contamination and then cooled. After cooling, the microorganisms are filled in packets. These packets are distributed to farmers.


Types of Biofertilizers

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Biofertilizers are broadly of three types:

  1. Bacteria
  2. Cyanobacteria
  3. Mycorrhizal fungi

Each of the three types can be further divided:

  1. Bacteria

  1. Free living nitrogen fixing bacteria: Example- Azotobacter, Rhodopseudomonas
  2. Loose association of nitrogen fixing bacteria: Example- Azospirillum
  3. Symbiotic nitrogen fixing bacteria: Example- Rhizobium
  1. Cyanobacteria

  1. Free living nitrogen fixing cyanobacteria: Example- Nostoc
  2. Symbiotic nitrogen fixing cyanobacteria: Example- Anabaena
  1. Mycorrhizal Fungi

  1. Ectomycorrhizae: Ectotrophic mycorrhizae
  2. Endomycorrhizae: Endotrophic mycorrhizae

Microbes

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Microbes or microorganisms are tiny living things that are too small to be seen by the naked eye and are found all around us. They can be found in water, soil, and air. They may exist as single-celled forms or as colonies of cells. The most common types of microbes are bacteria, viruses, protozoa, fungi.

Also Read: Evolution


Microbes as Biofertilizers

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Microorganisms used as biofertilizers:

  1. Azotobacter:

Azotobacter grows freely in the soil and enriches the soil with nitrogen. They are mostly saprophytic. Azotobacter helps reduce the use of man-made nitrogen fertilizers in paddy fields.

  1. Azospirillum:

Azospirillum grows in the rhizosphere of crop plants. They fix atmospheric nitrogen and transfer it to crop plants. This association is called associative mutualism.

  1. Rhizobium

Rhizobium forms symbiotic association with roots of higher plants. This can’t fix nitrogen as free organisms, but can only do that while in nodule due to the presence of pink pigments called leghaemoglobin. Leghaemoglobin removes oxygen which helps in nitrogen fixation. Ideally, crops should be grown in nitrogen free soil, otherwise, the ability of Rhizobium to fix nitrogen is hampered.

  1. Nostoc

These cyanobacteria or blue green algae grow freely in the soil. They are photosynthetic by nature and can enrich the soil with nitrogen.

  1. Anabaena

This nitrogen fixing cyanobacteria can develop a symbiotic association with liverworts, ferns and cycad roots. Anabaena provides nitrogen to the rhizosphere of paddy plants.

  1. Ectomycorrhizae

These fungi grow on the surface of the root and in the outer cortex of plants line Pinus. They help in absorption of water, minerals from the soil, production of antimicrobial substances in the rhizosphere.

  1. Endomycorrhizae

These fungi grow within the intercellular space of cortical regions of grasses, orchids. They produce a swollen area called a vesicle or finely branched masses called arbuscles. These fungi are also called vesicular arbuscular mycorrhizae. They help in absorption of mineral nutrition, release of growth promoting substances.


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  1. They increase the nitrogen and phosphorus available to crops more naturally than chemical fertilizers.
  2. They are simple to use, even for novice farmers.
  3. They do not pollute the soil or the environment, whereas chemical fertilizers often result in excessive input of nitrogen.
  4. They are a cheap, easy-to-use alternative to manufactured chemical fertilizers.
  5. They maintain normal soil fertility and make it biologically alive.
  6. They increase yield because of the nitrogen and phosphorus they add to the soil.

Also Read:


Disadvantages of Microbes as Biofertilizers

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  1. Since biofertilizers are alive microorganisms, they require special care for long-term storage. 
  2. If other microorganisms contaminate a particular biofertilizer or if the manufacturer uses the wrong strain, they become ineffective.
  3. The soil in itself should contain adequate nutrients for the microorganisms to thrive and work.
  4. Biofertilizers cannot totally replace conventional fertilizers.
  5. They lose their effectiveness if the soil is not moist.
  6. They are less effective if the soil is excessively acidic or alkaline
  7. The availability of some biofertilizers depends upon the availability of particular strains of microorganisms or culture media.

Things to Remember

  • Biofertilizers are living organisms, not chemicals.
  • Biofertilizers provide nitrogen and a few other nutrients. They don’t provide a whole spectrum of nutrition to crops.
  • Biofertilizers are essentially not connected with maintenance of air, water and texture of soil. They may affect them, but that’s not their primary purpose.
  • Our dependence on chemical fertilizers and pesticides sustained and encouraged the growth of chemical input industries which not only affected human health but also disturb the ecological balance.
  • Biofertilizers would play a key role in the productivity and sustainability of soil and protect the environment as eco-friendly and cost-effective inputs for the farmers.

Sample Questions

Ques. What are biofertilizers? (2 marks)

Ans. Biofertilizers are microorganisms that enrich the soil with nutrients, maximize the ecological benefits and minimize environmental impact. Biofertilizers increase the nutrient availability to crop plants either directly or through soil enrichment. The use of biofertilizers is advantageous mainly because it is environmentally friendly and sustainable. Azotobacter, Rhizobium, Azospirillum, Nostoc, Anabaena are common microbes used as biofertilizers. 

Ques. Why use microbes as biofertilizers? (3 marks)

Ans. We should use microbes as biofertilizers because they increase the nitrogen and phosphorus available to crops more naturally than chemical fertilizers. Moreover, they are simple to use, even for novice farmers. Biofertilizers do not pollute the environment, whereas chemical fertilizers often result in excessive input of nitrogen. Also, they are a cheap, easy-to-use alternative to manufactured chemical fertilizers. Apart from that, biofertilizers help crop plants develop stronger root systems. Crop plants resist stress better and live longer with the use of biofertilizers. Additionally, they maintain normal soil fertility and make it biologically alive.

Ques. Write about three microbes used as biofertilizers. (4 marks)

Ans. Bacteria, cyanobacteria and fungi are used as biofertilizers. Three such microbes which are used as biofertilizers are:

Rhizobium- Rhizobium forms symbiotic association with roots of higher plants. This can’t fix nitrogen as free organisms, but can only do that while in nodule due to the presence of pink pigments called leghaemoglobin. Leghaemoglobin removes oxygen which helps in nitrogen fixation. Ideally, crops should be grown in nitrogen free soil, otherwise, the ability of Rhizobium to fix nitrogen is hampered.

Nostoc- These cyanobacteria or blue green algae grow freely in the soil. They are photosynthetic by nature and can enrich the soil with nitrogen.

Anabaena- This nitrogen fixing cyanobacteria can develop a symbiotic association with liverworts, ferns and cycad roots. Anabaena provides nitrogen to the rhizosphere of paddy plants.

Ques. What are the issues faced regarding use of biofertilizers in India? (4 marks)

Ans. There are multiple issues regarding use of biofertilizers in India which should be dealt at multiple stakeholder levels. Some of them are-

  • Popularization of biofertilizers in India and supply to remote corners of the country is the most important issue that needs to be addressed.
  • Lack of knowledge of the farmers about biofertilizers.
  • Since yield using conventional fertilizers can be matched with yield using biofertilizers, farmers are reluctant.
  • Quality and standard of biofertilizer need to be high and uniform.

Ques. Describe the types of biofertilizers. (5 marks)

Ans. Out of many ways to classify biofertilizers, one of them is to broadly divide biofertilizers into bacteria, cyanobacteria and fungi.

  1. Bacteria
  • Free living nitrogen fixing bacteria: Example- Azotobacter, Rhodopseudomonas
  • Loose association of nitrogen fixing bacteria: Example- Azospirillum
  • Symbiotic nitrogen fixing bacteria: Example- Rhizobium
  1. Cyanobacteria
  • Free living nitrogen fixing cyanobacteria: Example- Nostoc
  • Symbiotic nitrogen fixing cyanobacteria: Example- Anabaena
  1. Mycorrhizal fungi
  • Ectomycorrhizae: Ectotrophic mycorrhizae
  • Endomycorrhizae: Endotrophic mycorrhizae

Ques. Write about the issues which discourages use of microbes as biofertilizers. (3 Marks)

Ans. Although biofertilizers have advantages like being environment friendly and sustainable, they are not without disadvantages. Biofertilizers are alive microorganisms, they require special care for long-term storage. There is a risk of biofertilizers getting contaminated by undesirable microorganisms. Sometimes, the soil is not suitable for use of biofertilizers.  Additionally, biofertilizers lose their effectiveness if the soil is not moist, and excessively acidic or alkaline. Lastly, the availability of some biofertilizers depends upon the availability of particular strains of microorganisms or culture media.

Ques. Fungi are used as biofertilizers. Elaborate. (4 Marks)

Ans. Fungi as biofertilizers can be divided in two classes depending upon the location of fungi in crop plant roots.

Ectomycorrhizae- These fungi grow on the surface of the root and in the outer cortex of plants line Pinus. They help in absorption of water, minerals from the soil, production of antimicrobial substances in the rhizosphere.

Endomycorrhizae- These fungi grow within the intercellular space of cortical regions of grasses, orchids. They produce a swollen area called a vesicle or finely branched masses called arbuscles. These fungi are also called vesicular arbuscular mycorrhizae. They help in absorption of mineral nutrition, release of growth promoting substances.

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