MHT CET PYQs for Biotechnology with Solutions: Practice MHT CET Previous Year Questions

Biotechnology is a Class 12 chapter in the Biology section of the MHT CET for candidates who are preparing for the PCB (Physics, Chemistry, and Biology).

The key topics covered in Biotechnology PYQs are:

Restriction enzymes and molecular scissors
PCR and in vitro amplification of a gene
Recombinant DNA technology
Industrial microbiology and fermentation
Biotechnology in agriculture and medicine

Practising Biotechnology MHT CET PYQ helps candidates to understand question pattern, improve calculation speed, and identify concepts which were asked in previous exams.

Based on the MHT CET Biology papers in the PDF, Biotechnology recorded 25 questions, and 14 of them came in the 2025 papers. Each Biology question carries 1 mark.

MHT CET Biotechnology Chapter: Question Count, Marks and Weightage:

What it shows	Number
Questions in the PDF (2024-2025)	25
Questions from the 2025 paper	14
Marks for each question	1

The PDF holds 25 questions from MHT CET 2025 to 2024, newest first, each with its exam date and shift. Options are as printed and answer keys are not included.

Biotechnology PYQ Statistics (2024-2025)

The table below shows the number of questions asked in MHT CET Biotechnology PYQs in each of the last five years.

Year	Questions Asked
2025	14
2024	11

The PDF holds Biology questions only from 2025 and 2024, and it has no Biology questions from other years.

Pair PYQs with Other Study Material for MHT CET 2027

Click on the link to practise problems and time management skills:

Resource	Link
MHT CET Sample Paper	Check Here
MHT CET Mock Test	Check Here

Question Style in MHT CET Biotechnology PYQs

Every question in this PDF is a single-correct MCQ. Most are theory questions on genetic engineering tools and on the uses of microbes in industry.

Question Style	What it Tests
Restriction enzymes	Which enzyme is considered molecular scissors, the type of enzyme that cuts DNA at different sites, and matching restriction enzymes with their recognition sequences
PCR and gene transfer	The technique used for in vitro amplification of a gene, and the temperatures used in the steps of PCR
Genetic engineering terms	What foreign DNA is called in genetic engineering, and how the host cell protects itself from viral DNA
Industrial microbiology	Which microbe is used to produce yoghurt, the use of Eremothecium ashbyi, and matching enzymes with the microbes that produce them
Applications	The protein formed by the gene from Bacillus thuringiensis, and the elimination of a defective gene by gene therapy

How to Use the Biotechnology PYQ PDF for MHT CET

Start with the 2025 papers first, because they hold 14 of the 25 questions.
Memorise the steps of PCR with the temperature for each, and the name of the enzyme used.
Make a table of enzymes and vitamins with the microbe that produces each, because match questions use them.
Solve year-wise PYQs by timing yourself, as time management is crucial.
Re-attempt only the questions you got wrong, and note the term or fact you missed.

Video: Biotechnology PYQs and MCQs

Source: GYAN LAB

Biotechnology PYQ for MHT CET Biology FAQs

Ques. How many questions come from Biotechnology every year?

Ans. In this PDF, the questions are 14 from 2025 and 11 from 2024. In 2025 the chapter had 14 questions.

Ques. What kind of questions are asked from Biotechnology?

Ans. Single-correct theory MCQs. Common ones ask for a tool of genetic engineering, a step of PCR, or a microbe used in industry.

Ques. What is the best way to practise Biotechnology PYQs?

Ans. First solve the 2025 questions completely, then the older ones, and finally re-attempt the questions you got wrong.

Ques. Can I score full marks from Biotechnology?

Ans. In 2025 the chapter had 14 questions. Learning the tools of genetic engineering and the industrial uses of microbes is enough to secure it. Each Biology question in MHT CET is worth 1 mark.

Ques. Does the Biotechnology PYQ PDF include solutions?

Ans. No. The PDF contains the questions with their exam date and shift, without answer keys or solutions.

MHT CET PYQs for Biotechnology with Solutions: Practice MHT CET Previous Year Questions

Biotechnology PYQ Statistics (2024-2025)

Pair PYQs with Other Study Material for MHT CET 2027

Question Style in MHT CET Biotechnology PYQs

How to Use the Biotechnology PYQ PDF for MHT CET

Video: Biotechnology PYQs and MCQs

Biotechnology PYQ for MHT CET Biology FAQs

1.
In a heterozygous tall pea plant, the possibility of T gamete fertilizing T gamete during its selfing is ___________%.

2.
Combination of foreign DNA and vector DNA are together called as _______.

3.
In recombinant DNA technology, which enzyme is used to cut DNA at specific recognition sites to produce restriction fragments?

4.
Hugo de Vries proposed ‘Mutation theory’ by studying the plant ______________.

5.
In the first step of PCR technique heating breaks the .......... bonds of DNA molecule.

6.
Which one of the following is a bacterial herbicide?

7.
The toxic substances released from Bacillus thuringiensis affect ........... of insect larva.

8.
Following are classified as plasma proteins EXCEPT ______________.

9.
In genetic engineering the Foreign DNA is called

10.
Water reaches to the top of trees of more than 100 feet against gravity, which one of the following is the main phenomenon responsible for this?

11.
Holandric genes are present on ______________.

12.
The \rule{1cm{0.15mm} gene from Bacillus thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.}

13.
The improved oil content and oil quality of oil crops like soybean oil palm rapeseed and sunflower have been achieved by the transfer of

14.
All of the following animals are uricotelic EXCEPT ______.

15.
Select the INCORRECT statement.

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Biotechnology PYQ Statistics (2024-2025)

Pair PYQs with Other Study Material for MHT CET 2027

Question Style in MHT CET Biotechnology PYQs

How to Use the Biotechnology PYQ PDF for MHT CET

Video: Biotechnology PYQs and MCQs

Biotechnology PYQ for MHT CET Biology FAQs

1.In a heterozygous tall pea plant, the possibility of T gamete fertilizing T gamete during its selfing is ___________%.

2.Combination of foreign DNA and vector DNA are together called as _______.

3.In recombinant DNA technology, which enzyme is used to cut DNA at specific recognition sites to produce restriction fragments?

4.Hugo de Vries proposed ‘Mutation theory’ by studying the plant ______________.

5.In the first step of PCR technique heating breaks the .......... bonds of DNA molecule.

6.Which one of the following is a bacterial herbicide?

7.The toxic substances released from Bacillus thuringiensis affect ........... of insect larva.

8.Following are classified as plasma proteins EXCEPT ______________.

9.In genetic engineering the Foreign DNA is called

10.Water reaches to the top of trees of more than 100 feet against gravity, which one of the following is the main phenomenon responsible for this?

11.Holandric genes are present on ______________.

12.The \rule{1cm{0.15mm} gene from Bacillus thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.}

13.The improved oil content and oil quality of oil crops like soybean oil palm rapeseed and sunflower have been achieved by the transfer of

14.All of the following animals are uricotelic EXCEPT ______.

15.Select the INCORRECT statement.

Comments

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1.
In a heterozygous tall pea plant, the possibility of T gamete fertilizing T gamete during its selfing is ___________%.

2.
Combination of foreign DNA and vector DNA are together called as _______.

3.
In recombinant DNA technology, which enzyme is used to cut DNA at specific recognition sites to produce restriction fragments?

4.
Hugo de Vries proposed ‘Mutation theory’ by studying the plant ______________.

5.
In the first step of PCR technique heating breaks the .......... bonds of DNA molecule.

6.
Which one of the following is a bacterial herbicide?

7.
The toxic substances released from Bacillus thuringiensis affect ........... of insect larva.

8.
Following are classified as plasma proteins EXCEPT ______________.

9.
In genetic engineering the Foreign DNA is called

10.
Water reaches to the top of trees of more than 100 feet against gravity, which one of the following is the main phenomenon responsible for this?

11.
Holandric genes are present on ______________.

12.
The \rule{1cm{0.15mm} gene from Bacillus thuringiensis produces a protein that forms crystalline inclusions in bacterial spores.}

13.
The improved oil content and oil quality of oil crops like soybean oil palm rapeseed and sunflower have been achieved by the transfer of

14.
All of the following animals are uricotelic EXCEPT ______.

15.
Select the INCORRECT statement.