The CUET 2025 exam is being conducted from 13th May to 3rd June. The CUET Biology Question Paper 2025 with Answer Key and Solution PDF is available here. The CUET Biology was moderate to difficult in terms of difficulty level.

As per the exam pattern, the CUET Biology exam consists of 50 questions for 250 marks to be attempted in 60 minutes. 5 marks are awarded for each correct answer, and 1 mark is deducted for incorrect answer.

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CUET 2025 Question Paper with Solution PDF

CUET Biology Question Paper 2025 with Answer Key

CUET Biology Question Paper 2025 Download PDF  Check Solution
CUET 2025 Biology
Question 1:

Which of the following organelles is involved in the synthesis of proteins?

  • (1) Ribosome
  • (2) Lysosome
  • (3) Golgi apparatus
  • (4) Peroxisome

Question 2:

If a heterozygous tall plant (Tt) is crossed with a dwarf plant (tt), what will be the phenotypic ratio of the offspring?

  • (1) 1 Tall : 3 Dwarf
  • (2) 1 Tall : 1 Dwarf
  • (3) All Tall
  • (4) All Dwarf

Question 3:

Which of the following elements is essential for the synthesis of chlorophyll?

  • (1) Calcium
  • (2) Magnesium
  • (3) Sodium
  • (4) Potassium

Question 4:

Which part of the nephron is primarily responsible for filtration of blood?

  • (1) Loop of Henle
  • (2) Proximal convoluted tubule
  • (3) Bowman's capsule
  • (4) Collecting duct

Question 5:

Which of the following is a pioneer species in primary succession on rocks?

  • (1) Ferns
  • (2) Lichens
  • (3) Mosses
  • (4) Algae

Question 6:

Which type of bond is found between amino acids in a protein chain?

  • (1) Hydrogen bond
  • (2) Peptide bond
  • (3) Ionic bond
  • (4) Glycosidic bond

Question 7:

Which enzyme is responsible for the unwinding of DNA during replication?

  • (1) DNA polymerase
  • (2) DNA ligase
  • (3) DNA helicase
  • (4) RNA primase

Question 8:

Double fertilization is a characteristic feature of which group of plants?

  • (1) Bryophytes
  • (2) Gymnosperms
  • (3) Pteridophytes
  • (4) Angiosperms

Question 9:

The part of the brain responsible for maintaining posture and balance is the:

  • (1) Cerebrum
  • (2) Medulla oblongata
  • (3) Cerebellum
  • (4) Hypothalamus

Question 10:

The gradual and predictable change in species composition in an area is known as:

  • (1) Succession
  • (2) Evolution
  • (3) Mutation
  • (4) Speciation

Question 11:

During which phase of mitosis do the centromeres split and chromatids move to opposite poles?

  • (1) Prophase
  • (2) Metaphase
  • (3) Anaphase
  • (4) Telophase

Question 12:

Which of the following organisms is correctly matched with its kingdom?

  • (1) Amoeba – Plantae
  • (2) Euglena - Fungi
  • (3) Paramecium - Protista
  • (4) Nostoc - Animalia

Question 13:

Which of the following is not a part of the human digestive system?

  • (1) Stomach
  • (2) Pancreas
  • (3) Liver
  • (4) Lungs

Question 14:

Which of the following is responsible for the transport of water in plants?

  • (1) Xylem
  • (2) Phloem
  • (3) Epidermis
  • (4) Stomata

Question 15:

Which gas is primarily responsible for the greenhouse effect?

  • (1) Oxygen
  • (2) Nitrogen
  • (3) Carbon dioxide
  • (4) Hydrogen

CUET Biology Important Topics and Difficulty Level

The CUET Biology 2025 question paper is expected to consist of 15–18 moderately difficult questions, covering topics such as Human Physiology, Reproduction, Cell Structure and Function, Plant Physiology, Genetics and Evolution, Biotechnology, and Biomolecules. 8–10 questions of easy to moderate difficulty are expected from topics like Ecology and Environment, Diversity in Living Organisms, and Biology and Human Welfare. The overall difficulty level of the CUET Biology paper is expected to be moderate to difficult.

Chapter Important Topics Expected Weightage (Questions)
Reproduction Human Reproduction, Reproductive Health, Menstrual Cycle, Fertilization 4 – 5
Genetics and Evolution Mendelian Inheritance, Molecular Basis of Inheritance, Evolution, Hardy-Weinberg Principle 3 – 4
Human Physiology Digestive System, Respiratory System, Circulatory System, Nervous System, Excretory System 4 – 5
Biotechnology Principles of Biotechnology, Genetic Engineering, Applications in Medicine 2 – 3
Ecology and Environment Ecosystems, Biodiversity, Environmental Issues, Conservation 2 – 3
Cell Structure and Function Cell Theory, Cell Organelles, Cell Division (Mitosis and Meiosis) 2 – 3
Plant Physiology Photosynthesis, Respiration, Plant Growth and Development, Transport in Plants 2 – 3
Biology and Human Welfare Human Diseases, Immunity, Microbes in Human Welfare, Cancer, AIDS 2 – 3
Diversity in Living Organisms Classification of Organisms, Five Kingdom Classification, Characteristics of Major Phyla 1 – 2
Biomolecules Carbohydrates, Proteins, Lipids, Nucleic Acids, Enzymes 1 – 2

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